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    ATCC cell lines hek 293t 17 atcc cat
    Cell Lines Hek 293t 17 Atcc Cat, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 12149 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Recombinant:

    Article Title: Interval between prior SARS-CoV-2 infection and booster vaccination impacts magnitude and quality of antibody and B cell responses.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Zombie NIR Fixable Viability Dye Biolegend Cat#423105 CellTrace CFSE Cell Proliferation Kit ThermoFisher Cat#C34554 R848 (Resiquimod) InvivoGen Cat#tlrl-r848-5 Human Recombinant IL-2 StemCell Technologies Cat#78036.2 1-Step Ultra TMB-ELISA Substrate Solution ThermoFisher Cat#34029 Stop Solution ThermoFisher Cat#SS04 Tween 20 Sigma Aldrich Cat#P1379 SARS-CoV-2-Nucleocapsid (N-terminal) NCI-Frederick Protein Expression Lab N/A Pooled Human Serum (collected prior to 2019) Lonza Cat#3P-105B MSD Blocker A Meso Scale Diagnostics Cat#R93AA MSD GOLD Read Buffer B Meso Scale Diagnostics Cat#R60AM MSD Diluent 100 Meso Scale Diagnostics Cat#R50AA Lipofectamine 3000 transfection reagent ThermoFisher Cat#L3000075 Reporter Gene Assay System Perkin Elmer Cat#6066769 Critical commercial assays Human Total IgG CBA Flex Set Bead C6 BD Biosciences Cat#558679 Human Ig CBA Master Buffer Kit BD Biosciences Cat#558683 V-Plex SARS-CoV-2 panel 25 (IgG) kit Meso Scale Diagnostics Cat#K15583U Deposited data Code for mixed-effect model statistical analysis This paper https://doi.org/10.5281/zenodo.7097099 Experimental models: Cell lines HEK-293T/17 ATCC CatCRL-11268; RRID: CVCL_1926 HEK-293T-ACE2 Michael Farzan and Huihui Mu, Scripps Research N/A Recombinant DNA VRC5601: pHR’CMV Luc (Naldini et al., 1996) N/A VRC5602: pCMV DR8.2 (Naldini et al., 1996) N/A VRC9260: TMPRSS2 Vaccine Research Center, NIH (DiPiazza et al., 2021) N/A Spike_SARS-CoV-2 D614G Vaccine Research Center, NIH (Corbett et al., 2021) N/A Spike_SARS-CoV-2 B.1.351 Vaccine Research Center, NIH (Corbett et al., 2021) N/A Spike_SARS-CoV-2 B.1.617.2 Vaccine Research Center, NIH (Corbett et al., 2021) N/A Spike_SARS-CoV-2 B.1.1.529 Vaccine Research Center, NIH (Gagne et al., 2022) N/A Software and algorithms GraphPad Prism v9.4 GraphPad RRID: SCR_002798 FlowJo v10.8.1 FlowJo RRID: SCR_008520 Labkey v19.2 Labkey N/A ll Article .. REAGENT or RESOURCE SOURCE IDENTIFIER Zombie NIR Fixable Viability Dye Biolegend Cat#423105 CellTrace CFSE Cell Proliferation Kit ThermoFisher Cat#C34554 R848 (Resiquimod) InvivoGen Cat#tlrl-r848-5 Human Recombinant IL-2 StemCell Technologies Cat#78036.2 1-Step Ultra TMB-ELISA Substrate Solution ThermoFisher Cat#34029 Stop Solution ThermoFisher Cat#SS04 Tween 20 Sigma Aldrich Cat#P1379 SARS-CoV-2-Nucleocapsid (N-terminal) NCI-Frederick Protein Expression Lab N/A Pooled Human Serum (collected prior to 2019) Lonza Cat#3P-105B MSD Blocker A Meso Scale Diagnostics Cat#R93AA MSD GOLD Read Buffer B Meso Scale Diagnostics Cat#R60AM MSD Diluent 100 Meso Scale Diagnostics Cat#R50AA Lipofectamine 3000 transfection reagent ThermoFisher Cat#L3000075 Reporter Gene Assay System Perkin Elmer Cat#6066769 Critical commercial assays Human Total IgG CBA Flex Set Bead C6 BD Biosciences Cat#558679 Human Ig CBA Master Buffer Kit BD Biosciences Cat#558683 V-Plex SARS-CoV-2 panel 25 (IgG) kit Meso Scale Diagnostics Cat#K15583U Deposited data Code for mixed-effect model statistical analysis This paper https://doi.org/10.5281/zenodo.7097099 Experimental models: Cell lines HEK-293T/17 ATCC Cat#CRL-11268; RRID: CVCL_1926 HEK-293T-ACE2 Michael Farzan and Huihui Mu, Scripps Research N/A Recombinant DNA VRC5601: pHR’CMV Luc (Naldini et al., 1996) N/A VRC5602: pCMV DR8.2 (Naldini et al., 1996) N/A VRC9260: TMPRSS2 Vaccine Research Center, NIH (DiPiazza et al., 2021) N/A Spike_SARS-CoV-2 D614G Vaccine Research Center, NIH (Corbett et al., 2021) N/A Spike_SARS-CoV-2 B.1.351 Vaccine Research Center, NIH (Corbett et al., 2021) N/A Spike_SARS-CoV-2 B.1.617.2 Vaccine Research Center, NIH (Corbett et al., 2021) N/A Spike_SARS-CoV-2 B.1.1.529 Vaccine Research Center, NIH (Gagne et al., 2022) N/A Software and algorithms GraphPad Prism v9.4 GraphPad RRID: SCR_002798 FlowJo v10.8.1 FlowJo RRID: SCR_008520 Labkey v19.2 Labkey N/A ll Article

    Article Title: Programmable iPSC-derived CAR-NK vesicles remodel the immune microenvironment and eradicate tumors.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Ultra-LEAFTM Purified anti-mouse IFN-γ Antibody Biolegend Cat#631054; RRID: AB_3097543 Brilliant Violet 421TM anti-mouse CD62L Antibody Biolegend Cat#104435; RRID: AB_10900082 Alexa Fluor® 700 anti-mouse CD44 Antibody Biolegend Cat#156009; RRID: AB_3068217 Anti-mouse CD8β (Lyt 3.2) Selleckchem Cat#A2137; RRID: AB_2687706 InVivoMAb anti-mouse PD-L1 BioXcell Cat#BE0101; RRID: AB_10949073 InVivoMAb anti-mouse CD47 BioXcell Cat#BE0270; RRID: AB_2687793 InVivoMAb anti-mouse CTLA-4 BioXcell Cat#BE0131; RRID: AB_10950184 Bacterial and virus strains BL21(DE3) Sigma-Aldrich 69450 T7 phage Novagen (EMD MIllipore) 70010–3 Chemicals, peptides, and recombinant proteins Puromycin Dihydrochloride Gibco Cat#A1113803 Mouse LIF Recombinant Protein Gibco Cat#PMC9484 Human SCF Recombinant Protein Gibco Cat#PHC2111 MethoCultTM GF M3434 Fisher scientific Cat#NC9099909 Mouse SCF Recombinant Protein PeproTech Cat#250-03-10UG Mouse IL-3 Recombinant Protein PeproTech Cat#213-13-10UG Human IL-6 Recombinant Protein PeproTech Cat#200-06-20UG Human IL-2 Recombinant Protein PeproTech Cat#200-02-50UG Mouse IL-15 Recombinant Protein PeproTech Cat#210-15-10UG Human VEGF-165 Recombinant Protein PeproTech Cat#100-20-10UG Human BMP-4 Recombinant Protein PeproTech Cat#120-05-5UG 2-Deoxy-D-glucose MCE Cat#HY-13966 PKH 67 MCE Cat#HY-D1421 DiR MCE Cat#HY-D1048 Matrigel Matrix Corning Cat#356234 Donkey serum Sigma-Aldrich Cat#D9663 Imidazole Sigma-Aldrich Cat#I202 Ni-NTA Agarose Qiagen Cat#30210 Imidazole Wash Buffer Bioworld Cat#42320000 RIPA buffer Beyotime Cat#P0013E Lenti-X concentrator Takara Cat#631231 methylcellulose Methocult Cat#M3434 Purified CD133 protein This paper N/A 89Zr Qilu hospital N/A Critical commercial assays Annexin V-FITC/PI Apoptosis Kit Biolegend Cat#640914 PKH67GL-1KT Sigma-Aldrich Cat#PKH67GL TUNEL Staining Kit Sigma-Aldrich Cat#APT110 L-Lactate Assay Kit Sigma-Aldrich Cat#MAK329 Glucose Uptake Assay Kit Abcam Cat#ab136956 Cell Counting Kit Yeasen Cat#40203ES60 BCA assay kit Beyotime Cat#P0009 Mouse IFN-γ ELISA Kit Beyotime Cat#PI508 Mycoplasma PCR Detection Kit Beyotime Cat#C0301S 10X Genomics Chromium NextGEM Single Cell 3′ Kit 10× Genomics Cat#PN-1000121 Luciferase Assay System Promega Cat#E1500 Mouse Ccl3 ELISA Kit JONLNBIO Cat#JL20414 (Continued on next page) e2 Cell Reports Medicine 7, 102545, February 17, 2026 Article ll OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Mouse Ccl5 ELISA Kit JONLNBIO Cat#JL17335 Mouse Cxcl1 ELISA Kit JONLNBIO Cat#JL20150 Mouse Cxcl5 ELISA Kit JONLNBIO Cat#JL28925 Mouse IL-1α ELISA Kit JONLNBIO Cat#JL13044 Mouse IL-1β ELISA Kit JONLNBIO Cat#JL18442 Mouse IL-6 ELISA Kit JONLNBIO Cat#JL20268 Mouse Tnf-α ELISA Kit JONLNBIO Cat#JL10484 Mouse Csf3 ELISA Kit JONLNBIO Cat#JL10697 Mouse Cxcl2 ELISA Kit Abnova Cat#KA1505 Deposited data Single-cell sequencing data This paper GEO: GSE293929 Experimental models: Cell lines HEK 293T/17 ATCC CatCRL-11268 K562 ATCC Cat#CCL-243 Panc02 ATCC Cat#CRL-2553 Mouse iPSC Alstem Cat#iPS02M Human iPSC ATCC Cat#ACS-1027 H22 Ubigene Biosciences Cat#YC-C053 Colon26 Creative biogene Cat#CSC-RR0461 Hep-53.4 CLS Cell Lines Service Cat#400200 Panc02-Luc This paper N/A Colon26-Luc This paper N/A Hep-53.4-Luc This paper N/A Experimental models: Organisms/strains F4/80-Cre mice The Jackson laboratory N/A huHSC-NCG-M Gempharmatech Cat#T057533 BALB/c Mice Charles Cat#028 C57BL/6 Mice Charles Cat#027 C57BL/6Smoc-Nos2em1(flox)Smoc Shanghai Model Organisms Cat#NM-CKO-226494 Macrophage-specific Nos2-KO mice This paper N/A Recombinant DNA pCDH-CMV-MCS-EF1α-GreenPuro System Biosciences Cat#CD513B-1 psPAX2 Addgene Cat#12260 pMD2.G Addgene Cat#12259 pLVX-mbIL-21-puro Addgene Cat#125839 Luciferase lentivirus Applied Biological Materials Cat#LVP010079 pET-14B-CD133 This paper N/A Software and algorithms ImageJ Schneider et al.45 https://imagej.net/ij/ Graphpad Prism version 10.0 GraphPad software https://www.graphpad.com FlowJo 10.0 FlowJo, LLC https://www.flowjo.com/ Living Image software v4.5 PerkinElmer N/A SPSS 20.0 IBM https://www.ibm.com/it-it/products/ spss-statistics Biacore S200 Evaluation software Cytiva https://www.cytivalifesciences.com/en/ us/support/software/biacore-downloads/ Biorender Biorender https://app.biorender.com/ Other Nanobodies targeting CD133 This paper N/A Cell Reports Medicine 7, 102545, February 17, 2026 e3 Article ll OPEN ACCESS .. Immunocompetent male C57BL/6 (Cat. 027) and male BALB/c (Cat. 028) mice, aged 8 weeks, were acquired commercially (Charles River Laboratories, IL, USA).

    Article Title: Phenotypic heterogeneity defines B cell responses to repeated SARS-CoV-2 exposures through vaccination and infection.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Deposited data Original/source data for all figures and the original codes for statistical analysis This paper https://doi.org/10.5281/zenodo.13350229 Experimental models: Cell lines HEK-293T/17 ATCC CatCRL-11268; RRID: CVCL_1926 HEK-293T-ACE2 Michael Farzan & Huihui Mu, Scripps Research N/A Recombinant DNA VRC5601: pHR’CMV Luc I.M. ..

    Expressing:

    Article Title: Interval between prior SARS-CoV-2 infection and booster vaccination impacts magnitude and quality of antibody and B cell responses.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Zombie NIR Fixable Viability Dye Biolegend Cat#423105 CellTrace CFSE Cell Proliferation Kit ThermoFisher Cat#C34554 R848 (Resiquimod) InvivoGen Cat#tlrl-r848-5 Human Recombinant IL-2 StemCell Technologies Cat#78036.2 1-Step Ultra TMB-ELISA Substrate Solution ThermoFisher Cat#34029 Stop Solution ThermoFisher Cat#SS04 Tween 20 Sigma Aldrich Cat#P1379 SARS-CoV-2-Nucleocapsid (N-terminal) NCI-Frederick Protein Expression Lab N/A Pooled Human Serum (collected prior to 2019) Lonza Cat#3P-105B MSD Blocker A Meso Scale Diagnostics Cat#R93AA MSD GOLD Read Buffer B Meso Scale Diagnostics Cat#R60AM MSD Diluent 100 Meso Scale Diagnostics Cat#R50AA Lipofectamine 3000 transfection reagent ThermoFisher Cat#L3000075 Reporter Gene Assay System Perkin Elmer Cat#6066769 Critical commercial assays Human Total IgG CBA Flex Set Bead C6 BD Biosciences Cat#558679 Human Ig CBA Master Buffer Kit BD Biosciences Cat#558683 V-Plex SARS-CoV-2 panel 25 (IgG) kit Meso Scale Diagnostics Cat#K15583U Deposited data Code for mixed-effect model statistical analysis This paper https://doi.org/10.5281/zenodo.7097099 Experimental models: Cell lines HEK-293T/17 ATCC CatCRL-11268; RRID: CVCL_1926 HEK-293T-ACE2 Michael Farzan and Huihui Mu, Scripps Research N/A Recombinant DNA VRC5601: pHR’CMV Luc (Naldini et al., 1996) N/A VRC5602: pCMV DR8.2 (Naldini et al., 1996) N/A VRC9260: TMPRSS2 Vaccine Research Center, NIH (DiPiazza et al., 2021) N/A Spike_SARS-CoV-2 D614G Vaccine Research Center, NIH (Corbett et al., 2021) N/A Spike_SARS-CoV-2 B.1.351 Vaccine Research Center, NIH (Corbett et al., 2021) N/A Spike_SARS-CoV-2 B.1.617.2 Vaccine Research Center, NIH (Corbett et al., 2021) N/A Spike_SARS-CoV-2 B.1.1.529 Vaccine Research Center, NIH (Gagne et al., 2022) N/A Software and algorithms GraphPad Prism v9.4 GraphPad RRID: SCR_002798 FlowJo v10.8.1 FlowJo RRID: SCR_008520 Labkey v19.2 Labkey N/A ll Article .. REAGENT or RESOURCE SOURCE IDENTIFIER Zombie NIR Fixable Viability Dye Biolegend Cat#423105 CellTrace CFSE Cell Proliferation Kit ThermoFisher Cat#C34554 R848 (Resiquimod) InvivoGen Cat#tlrl-r848-5 Human Recombinant IL-2 StemCell Technologies Cat#78036.2 1-Step Ultra TMB-ELISA Substrate Solution ThermoFisher Cat#34029 Stop Solution ThermoFisher Cat#SS04 Tween 20 Sigma Aldrich Cat#P1379 SARS-CoV-2-Nucleocapsid (N-terminal) NCI-Frederick Protein Expression Lab N/A Pooled Human Serum (collected prior to 2019) Lonza Cat#3P-105B MSD Blocker A Meso Scale Diagnostics Cat#R93AA MSD GOLD Read Buffer B Meso Scale Diagnostics Cat#R60AM MSD Diluent 100 Meso Scale Diagnostics Cat#R50AA Lipofectamine 3000 transfection reagent ThermoFisher Cat#L3000075 Reporter Gene Assay System Perkin Elmer Cat#6066769 Critical commercial assays Human Total IgG CBA Flex Set Bead C6 BD Biosciences Cat#558679 Human Ig CBA Master Buffer Kit BD Biosciences Cat#558683 V-Plex SARS-CoV-2 panel 25 (IgG) kit Meso Scale Diagnostics Cat#K15583U Deposited data Code for mixed-effect model statistical analysis This paper https://doi.org/10.5281/zenodo.7097099 Experimental models: Cell lines HEK-293T/17 ATCC Cat#CRL-11268; RRID: CVCL_1926 HEK-293T-ACE2 Michael Farzan and Huihui Mu, Scripps Research N/A Recombinant DNA VRC5601: pHR’CMV Luc (Naldini et al., 1996) N/A VRC5602: pCMV DR8.2 (Naldini et al., 1996) N/A VRC9260: TMPRSS2 Vaccine Research Center, NIH (DiPiazza et al., 2021) N/A Spike_SARS-CoV-2 D614G Vaccine Research Center, NIH (Corbett et al., 2021) N/A Spike_SARS-CoV-2 B.1.351 Vaccine Research Center, NIH (Corbett et al., 2021) N/A Spike_SARS-CoV-2 B.1.617.2 Vaccine Research Center, NIH (Corbett et al., 2021) N/A Spike_SARS-CoV-2 B.1.1.529 Vaccine Research Center, NIH (Gagne et al., 2022) N/A Software and algorithms GraphPad Prism v9.4 GraphPad RRID: SCR_002798 FlowJo v10.8.1 FlowJo RRID: SCR_008520 Labkey v19.2 Labkey N/A ll Article

    Transfection:

    Article Title: Interval between prior SARS-CoV-2 infection and booster vaccination impacts magnitude and quality of antibody and B cell responses.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Zombie NIR Fixable Viability Dye Biolegend Cat#423105 CellTrace CFSE Cell Proliferation Kit ThermoFisher Cat#C34554 R848 (Resiquimod) InvivoGen Cat#tlrl-r848-5 Human Recombinant IL-2 StemCell Technologies Cat#78036.2 1-Step Ultra TMB-ELISA Substrate Solution ThermoFisher Cat#34029 Stop Solution ThermoFisher Cat#SS04 Tween 20 Sigma Aldrich Cat#P1379 SARS-CoV-2-Nucleocapsid (N-terminal) NCI-Frederick Protein Expression Lab N/A Pooled Human Serum (collected prior to 2019) Lonza Cat#3P-105B MSD Blocker A Meso Scale Diagnostics Cat#R93AA MSD GOLD Read Buffer B Meso Scale Diagnostics Cat#R60AM MSD Diluent 100 Meso Scale Diagnostics Cat#R50AA Lipofectamine 3000 transfection reagent ThermoFisher Cat#L3000075 Reporter Gene Assay System Perkin Elmer Cat#6066769 Critical commercial assays Human Total IgG CBA Flex Set Bead C6 BD Biosciences Cat#558679 Human Ig CBA Master Buffer Kit BD Biosciences Cat#558683 V-Plex SARS-CoV-2 panel 25 (IgG) kit Meso Scale Diagnostics Cat#K15583U Deposited data Code for mixed-effect model statistical analysis This paper https://doi.org/10.5281/zenodo.7097099 Experimental models: Cell lines HEK-293T/17 ATCC CatCRL-11268; RRID: CVCL_1926 HEK-293T-ACE2 Michael Farzan and Huihui Mu, Scripps Research N/A Recombinant DNA VRC5601: pHR’CMV Luc (Naldini et al., 1996) N/A VRC5602: pCMV DR8.2 (Naldini et al., 1996) N/A VRC9260: TMPRSS2 Vaccine Research Center, NIH (DiPiazza et al., 2021) N/A Spike_SARS-CoV-2 D614G Vaccine Research Center, NIH (Corbett et al., 2021) N/A Spike_SARS-CoV-2 B.1.351 Vaccine Research Center, NIH (Corbett et al., 2021) N/A Spike_SARS-CoV-2 B.1.617.2 Vaccine Research Center, NIH (Corbett et al., 2021) N/A Spike_SARS-CoV-2 B.1.1.529 Vaccine Research Center, NIH (Gagne et al., 2022) N/A Software and algorithms GraphPad Prism v9.4 GraphPad RRID: SCR_002798 FlowJo v10.8.1 FlowJo RRID: SCR_008520 Labkey v19.2 Labkey N/A ll Article .. REAGENT or RESOURCE SOURCE IDENTIFIER Zombie NIR Fixable Viability Dye Biolegend Cat#423105 CellTrace CFSE Cell Proliferation Kit ThermoFisher Cat#C34554 R848 (Resiquimod) InvivoGen Cat#tlrl-r848-5 Human Recombinant IL-2 StemCell Technologies Cat#78036.2 1-Step Ultra TMB-ELISA Substrate Solution ThermoFisher Cat#34029 Stop Solution ThermoFisher Cat#SS04 Tween 20 Sigma Aldrich Cat#P1379 SARS-CoV-2-Nucleocapsid (N-terminal) NCI-Frederick Protein Expression Lab N/A Pooled Human Serum (collected prior to 2019) Lonza Cat#3P-105B MSD Blocker A Meso Scale Diagnostics Cat#R93AA MSD GOLD Read Buffer B Meso Scale Diagnostics Cat#R60AM MSD Diluent 100 Meso Scale Diagnostics Cat#R50AA Lipofectamine 3000 transfection reagent ThermoFisher Cat#L3000075 Reporter Gene Assay System Perkin Elmer Cat#6066769 Critical commercial assays Human Total IgG CBA Flex Set Bead C6 BD Biosciences Cat#558679 Human Ig CBA Master Buffer Kit BD Biosciences Cat#558683 V-Plex SARS-CoV-2 panel 25 (IgG) kit Meso Scale Diagnostics Cat#K15583U Deposited data Code for mixed-effect model statistical analysis This paper https://doi.org/10.5281/zenodo.7097099 Experimental models: Cell lines HEK-293T/17 ATCC Cat#CRL-11268; RRID: CVCL_1926 HEK-293T-ACE2 Michael Farzan and Huihui Mu, Scripps Research N/A Recombinant DNA VRC5601: pHR’CMV Luc (Naldini et al., 1996) N/A VRC5602: pCMV DR8.2 (Naldini et al., 1996) N/A VRC9260: TMPRSS2 Vaccine Research Center, NIH (DiPiazza et al., 2021) N/A Spike_SARS-CoV-2 D614G Vaccine Research Center, NIH (Corbett et al., 2021) N/A Spike_SARS-CoV-2 B.1.351 Vaccine Research Center, NIH (Corbett et al., 2021) N/A Spike_SARS-CoV-2 B.1.617.2 Vaccine Research Center, NIH (Corbett et al., 2021) N/A Spike_SARS-CoV-2 B.1.1.529 Vaccine Research Center, NIH (Gagne et al., 2022) N/A Software and algorithms GraphPad Prism v9.4 GraphPad RRID: SCR_002798 FlowJo v10.8.1 FlowJo RRID: SCR_008520 Labkey v19.2 Labkey N/A ll Article

    Reporter Gene Assay:

    Article Title: Interval between prior SARS-CoV-2 infection and booster vaccination impacts magnitude and quality of antibody and B cell responses.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Zombie NIR Fixable Viability Dye Biolegend Cat#423105 CellTrace CFSE Cell Proliferation Kit ThermoFisher Cat#C34554 R848 (Resiquimod) InvivoGen Cat#tlrl-r848-5 Human Recombinant IL-2 StemCell Technologies Cat#78036.2 1-Step Ultra TMB-ELISA Substrate Solution ThermoFisher Cat#34029 Stop Solution ThermoFisher Cat#SS04 Tween 20 Sigma Aldrich Cat#P1379 SARS-CoV-2-Nucleocapsid (N-terminal) NCI-Frederick Protein Expression Lab N/A Pooled Human Serum (collected prior to 2019) Lonza Cat#3P-105B MSD Blocker A Meso Scale Diagnostics Cat#R93AA MSD GOLD Read Buffer B Meso Scale Diagnostics Cat#R60AM MSD Diluent 100 Meso Scale Diagnostics Cat#R50AA Lipofectamine 3000 transfection reagent ThermoFisher Cat#L3000075 Reporter Gene Assay System Perkin Elmer Cat#6066769 Critical commercial assays Human Total IgG CBA Flex Set Bead C6 BD Biosciences Cat#558679 Human Ig CBA Master Buffer Kit BD Biosciences Cat#558683 V-Plex SARS-CoV-2 panel 25 (IgG) kit Meso Scale Diagnostics Cat#K15583U Deposited data Code for mixed-effect model statistical analysis This paper https://doi.org/10.5281/zenodo.7097099 Experimental models: Cell lines HEK-293T/17 ATCC CatCRL-11268; RRID: CVCL_1926 HEK-293T-ACE2 Michael Farzan and Huihui Mu, Scripps Research N/A Recombinant DNA VRC5601: pHR’CMV Luc (Naldini et al., 1996) N/A VRC5602: pCMV DR8.2 (Naldini et al., 1996) N/A VRC9260: TMPRSS2 Vaccine Research Center, NIH (DiPiazza et al., 2021) N/A Spike_SARS-CoV-2 D614G Vaccine Research Center, NIH (Corbett et al., 2021) N/A Spike_SARS-CoV-2 B.1.351 Vaccine Research Center, NIH (Corbett et al., 2021) N/A Spike_SARS-CoV-2 B.1.617.2 Vaccine Research Center, NIH (Corbett et al., 2021) N/A Spike_SARS-CoV-2 B.1.1.529 Vaccine Research Center, NIH (Gagne et al., 2022) N/A Software and algorithms GraphPad Prism v9.4 GraphPad RRID: SCR_002798 FlowJo v10.8.1 FlowJo RRID: SCR_008520 Labkey v19.2 Labkey N/A ll Article .. REAGENT or RESOURCE SOURCE IDENTIFIER Zombie NIR Fixable Viability Dye Biolegend Cat#423105 CellTrace CFSE Cell Proliferation Kit ThermoFisher Cat#C34554 R848 (Resiquimod) InvivoGen Cat#tlrl-r848-5 Human Recombinant IL-2 StemCell Technologies Cat#78036.2 1-Step Ultra TMB-ELISA Substrate Solution ThermoFisher Cat#34029 Stop Solution ThermoFisher Cat#SS04 Tween 20 Sigma Aldrich Cat#P1379 SARS-CoV-2-Nucleocapsid (N-terminal) NCI-Frederick Protein Expression Lab N/A Pooled Human Serum (collected prior to 2019) Lonza Cat#3P-105B MSD Blocker A Meso Scale Diagnostics Cat#R93AA MSD GOLD Read Buffer B Meso Scale Diagnostics Cat#R60AM MSD Diluent 100 Meso Scale Diagnostics Cat#R50AA Lipofectamine 3000 transfection reagent ThermoFisher Cat#L3000075 Reporter Gene Assay System Perkin Elmer Cat#6066769 Critical commercial assays Human Total IgG CBA Flex Set Bead C6 BD Biosciences Cat#558679 Human Ig CBA Master Buffer Kit BD Biosciences Cat#558683 V-Plex SARS-CoV-2 panel 25 (IgG) kit Meso Scale Diagnostics Cat#K15583U Deposited data Code for mixed-effect model statistical analysis This paper https://doi.org/10.5281/zenodo.7097099 Experimental models: Cell lines HEK-293T/17 ATCC Cat#CRL-11268; RRID: CVCL_1926 HEK-293T-ACE2 Michael Farzan and Huihui Mu, Scripps Research N/A Recombinant DNA VRC5601: pHR’CMV Luc (Naldini et al., 1996) N/A VRC5602: pCMV DR8.2 (Naldini et al., 1996) N/A VRC9260: TMPRSS2 Vaccine Research Center, NIH (DiPiazza et al., 2021) N/A Spike_SARS-CoV-2 D614G Vaccine Research Center, NIH (Corbett et al., 2021) N/A Spike_SARS-CoV-2 B.1.351 Vaccine Research Center, NIH (Corbett et al., 2021) N/A Spike_SARS-CoV-2 B.1.617.2 Vaccine Research Center, NIH (Corbett et al., 2021) N/A Spike_SARS-CoV-2 B.1.1.529 Vaccine Research Center, NIH (Gagne et al., 2022) N/A Software and algorithms GraphPad Prism v9.4 GraphPad RRID: SCR_002798 FlowJo v10.8.1 FlowJo RRID: SCR_008520 Labkey v19.2 Labkey N/A ll Article

    Crocin Bleaching Assay:

    Article Title: Interval between prior SARS-CoV-2 infection and booster vaccination impacts magnitude and quality of antibody and B cell responses.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Zombie NIR Fixable Viability Dye Biolegend Cat#423105 CellTrace CFSE Cell Proliferation Kit ThermoFisher Cat#C34554 R848 (Resiquimod) InvivoGen Cat#tlrl-r848-5 Human Recombinant IL-2 StemCell Technologies Cat#78036.2 1-Step Ultra TMB-ELISA Substrate Solution ThermoFisher Cat#34029 Stop Solution ThermoFisher Cat#SS04 Tween 20 Sigma Aldrich Cat#P1379 SARS-CoV-2-Nucleocapsid (N-terminal) NCI-Frederick Protein Expression Lab N/A Pooled Human Serum (collected prior to 2019) Lonza Cat#3P-105B MSD Blocker A Meso Scale Diagnostics Cat#R93AA MSD GOLD Read Buffer B Meso Scale Diagnostics Cat#R60AM MSD Diluent 100 Meso Scale Diagnostics Cat#R50AA Lipofectamine 3000 transfection reagent ThermoFisher Cat#L3000075 Reporter Gene Assay System Perkin Elmer Cat#6066769 Critical commercial assays Human Total IgG CBA Flex Set Bead C6 BD Biosciences Cat#558679 Human Ig CBA Master Buffer Kit BD Biosciences Cat#558683 V-Plex SARS-CoV-2 panel 25 (IgG) kit Meso Scale Diagnostics Cat#K15583U Deposited data Code for mixed-effect model statistical analysis This paper https://doi.org/10.5281/zenodo.7097099 Experimental models: Cell lines HEK-293T/17 ATCC CatCRL-11268; RRID: CVCL_1926 HEK-293T-ACE2 Michael Farzan and Huihui Mu, Scripps Research N/A Recombinant DNA VRC5601: pHR’CMV Luc (Naldini et al., 1996) N/A VRC5602: pCMV DR8.2 (Naldini et al., 1996) N/A VRC9260: TMPRSS2 Vaccine Research Center, NIH (DiPiazza et al., 2021) N/A Spike_SARS-CoV-2 D614G Vaccine Research Center, NIH (Corbett et al., 2021) N/A Spike_SARS-CoV-2 B.1.351 Vaccine Research Center, NIH (Corbett et al., 2021) N/A Spike_SARS-CoV-2 B.1.617.2 Vaccine Research Center, NIH (Corbett et al., 2021) N/A Spike_SARS-CoV-2 B.1.1.529 Vaccine Research Center, NIH (Gagne et al., 2022) N/A Software and algorithms GraphPad Prism v9.4 GraphPad RRID: SCR_002798 FlowJo v10.8.1 FlowJo RRID: SCR_008520 Labkey v19.2 Labkey N/A ll Article .. REAGENT or RESOURCE SOURCE IDENTIFIER Zombie NIR Fixable Viability Dye Biolegend Cat#423105 CellTrace CFSE Cell Proliferation Kit ThermoFisher Cat#C34554 R848 (Resiquimod) InvivoGen Cat#tlrl-r848-5 Human Recombinant IL-2 StemCell Technologies Cat#78036.2 1-Step Ultra TMB-ELISA Substrate Solution ThermoFisher Cat#34029 Stop Solution ThermoFisher Cat#SS04 Tween 20 Sigma Aldrich Cat#P1379 SARS-CoV-2-Nucleocapsid (N-terminal) NCI-Frederick Protein Expression Lab N/A Pooled Human Serum (collected prior to 2019) Lonza Cat#3P-105B MSD Blocker A Meso Scale Diagnostics Cat#R93AA MSD GOLD Read Buffer B Meso Scale Diagnostics Cat#R60AM MSD Diluent 100 Meso Scale Diagnostics Cat#R50AA Lipofectamine 3000 transfection reagent ThermoFisher Cat#L3000075 Reporter Gene Assay System Perkin Elmer Cat#6066769 Critical commercial assays Human Total IgG CBA Flex Set Bead C6 BD Biosciences Cat#558679 Human Ig CBA Master Buffer Kit BD Biosciences Cat#558683 V-Plex SARS-CoV-2 panel 25 (IgG) kit Meso Scale Diagnostics Cat#K15583U Deposited data Code for mixed-effect model statistical analysis This paper https://doi.org/10.5281/zenodo.7097099 Experimental models: Cell lines HEK-293T/17 ATCC Cat#CRL-11268; RRID: CVCL_1926 HEK-293T-ACE2 Michael Farzan and Huihui Mu, Scripps Research N/A Recombinant DNA VRC5601: pHR’CMV Luc (Naldini et al., 1996) N/A VRC5602: pCMV DR8.2 (Naldini et al., 1996) N/A VRC9260: TMPRSS2 Vaccine Research Center, NIH (DiPiazza et al., 2021) N/A Spike_SARS-CoV-2 D614G Vaccine Research Center, NIH (Corbett et al., 2021) N/A Spike_SARS-CoV-2 B.1.351 Vaccine Research Center, NIH (Corbett et al., 2021) N/A Spike_SARS-CoV-2 B.1.617.2 Vaccine Research Center, NIH (Corbett et al., 2021) N/A Spike_SARS-CoV-2 B.1.1.529 Vaccine Research Center, NIH (Gagne et al., 2022) N/A Software and algorithms GraphPad Prism v9.4 GraphPad RRID: SCR_002798 FlowJo v10.8.1 FlowJo RRID: SCR_008520 Labkey v19.2 Labkey N/A ll Article

    Software:

    Article Title: Interval between prior SARS-CoV-2 infection and booster vaccination impacts magnitude and quality of antibody and B cell responses.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Zombie NIR Fixable Viability Dye Biolegend Cat#423105 CellTrace CFSE Cell Proliferation Kit ThermoFisher Cat#C34554 R848 (Resiquimod) InvivoGen Cat#tlrl-r848-5 Human Recombinant IL-2 StemCell Technologies Cat#78036.2 1-Step Ultra TMB-ELISA Substrate Solution ThermoFisher Cat#34029 Stop Solution ThermoFisher Cat#SS04 Tween 20 Sigma Aldrich Cat#P1379 SARS-CoV-2-Nucleocapsid (N-terminal) NCI-Frederick Protein Expression Lab N/A Pooled Human Serum (collected prior to 2019) Lonza Cat#3P-105B MSD Blocker A Meso Scale Diagnostics Cat#R93AA MSD GOLD Read Buffer B Meso Scale Diagnostics Cat#R60AM MSD Diluent 100 Meso Scale Diagnostics Cat#R50AA Lipofectamine 3000 transfection reagent ThermoFisher Cat#L3000075 Reporter Gene Assay System Perkin Elmer Cat#6066769 Critical commercial assays Human Total IgG CBA Flex Set Bead C6 BD Biosciences Cat#558679 Human Ig CBA Master Buffer Kit BD Biosciences Cat#558683 V-Plex SARS-CoV-2 panel 25 (IgG) kit Meso Scale Diagnostics Cat#K15583U Deposited data Code for mixed-effect model statistical analysis This paper https://doi.org/10.5281/zenodo.7097099 Experimental models: Cell lines HEK-293T/17 ATCC CatCRL-11268; RRID: CVCL_1926 HEK-293T-ACE2 Michael Farzan and Huihui Mu, Scripps Research N/A Recombinant DNA VRC5601: pHR’CMV Luc (Naldini et al., 1996) N/A VRC5602: pCMV DR8.2 (Naldini et al., 1996) N/A VRC9260: TMPRSS2 Vaccine Research Center, NIH (DiPiazza et al., 2021) N/A Spike_SARS-CoV-2 D614G Vaccine Research Center, NIH (Corbett et al., 2021) N/A Spike_SARS-CoV-2 B.1.351 Vaccine Research Center, NIH (Corbett et al., 2021) N/A Spike_SARS-CoV-2 B.1.617.2 Vaccine Research Center, NIH (Corbett et al., 2021) N/A Spike_SARS-CoV-2 B.1.1.529 Vaccine Research Center, NIH (Gagne et al., 2022) N/A Software and algorithms GraphPad Prism v9.4 GraphPad RRID: SCR_002798 FlowJo v10.8.1 FlowJo RRID: SCR_008520 Labkey v19.2 Labkey N/A ll Article .. REAGENT or RESOURCE SOURCE IDENTIFIER Zombie NIR Fixable Viability Dye Biolegend Cat#423105 CellTrace CFSE Cell Proliferation Kit ThermoFisher Cat#C34554 R848 (Resiquimod) InvivoGen Cat#tlrl-r848-5 Human Recombinant IL-2 StemCell Technologies Cat#78036.2 1-Step Ultra TMB-ELISA Substrate Solution ThermoFisher Cat#34029 Stop Solution ThermoFisher Cat#SS04 Tween 20 Sigma Aldrich Cat#P1379 SARS-CoV-2-Nucleocapsid (N-terminal) NCI-Frederick Protein Expression Lab N/A Pooled Human Serum (collected prior to 2019) Lonza Cat#3P-105B MSD Blocker A Meso Scale Diagnostics Cat#R93AA MSD GOLD Read Buffer B Meso Scale Diagnostics Cat#R60AM MSD Diluent 100 Meso Scale Diagnostics Cat#R50AA Lipofectamine 3000 transfection reagent ThermoFisher Cat#L3000075 Reporter Gene Assay System Perkin Elmer Cat#6066769 Critical commercial assays Human Total IgG CBA Flex Set Bead C6 BD Biosciences Cat#558679 Human Ig CBA Master Buffer Kit BD Biosciences Cat#558683 V-Plex SARS-CoV-2 panel 25 (IgG) kit Meso Scale Diagnostics Cat#K15583U Deposited data Code for mixed-effect model statistical analysis This paper https://doi.org/10.5281/zenodo.7097099 Experimental models: Cell lines HEK-293T/17 ATCC Cat#CRL-11268; RRID: CVCL_1926 HEK-293T-ACE2 Michael Farzan and Huihui Mu, Scripps Research N/A Recombinant DNA VRC5601: pHR’CMV Luc (Naldini et al., 1996) N/A VRC5602: pCMV DR8.2 (Naldini et al., 1996) N/A VRC9260: TMPRSS2 Vaccine Research Center, NIH (DiPiazza et al., 2021) N/A Spike_SARS-CoV-2 D614G Vaccine Research Center, NIH (Corbett et al., 2021) N/A Spike_SARS-CoV-2 B.1.351 Vaccine Research Center, NIH (Corbett et al., 2021) N/A Spike_SARS-CoV-2 B.1.617.2 Vaccine Research Center, NIH (Corbett et al., 2021) N/A Spike_SARS-CoV-2 B.1.1.529 Vaccine Research Center, NIH (Gagne et al., 2022) N/A Software and algorithms GraphPad Prism v9.4 GraphPad RRID: SCR_002798 FlowJo v10.8.1 FlowJo RRID: SCR_008520 Labkey v19.2 Labkey N/A ll Article

    Article Title: Programmable iPSC-derived CAR-NK vesicles remodel the immune microenvironment and eradicate tumors.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Ultra-LEAFTM Purified anti-mouse IFN-γ Antibody Biolegend Cat#631054; RRID: AB_3097543 Brilliant Violet 421TM anti-mouse CD62L Antibody Biolegend Cat#104435; RRID: AB_10900082 Alexa Fluor® 700 anti-mouse CD44 Antibody Biolegend Cat#156009; RRID: AB_3068217 Anti-mouse CD8β (Lyt 3.2) Selleckchem Cat#A2137; RRID: AB_2687706 InVivoMAb anti-mouse PD-L1 BioXcell Cat#BE0101; RRID: AB_10949073 InVivoMAb anti-mouse CD47 BioXcell Cat#BE0270; RRID: AB_2687793 InVivoMAb anti-mouse CTLA-4 BioXcell Cat#BE0131; RRID: AB_10950184 Bacterial and virus strains BL21(DE3) Sigma-Aldrich 69450 T7 phage Novagen (EMD MIllipore) 70010–3 Chemicals, peptides, and recombinant proteins Puromycin Dihydrochloride Gibco Cat#A1113803 Mouse LIF Recombinant Protein Gibco Cat#PMC9484 Human SCF Recombinant Protein Gibco Cat#PHC2111 MethoCultTM GF M3434 Fisher scientific Cat#NC9099909 Mouse SCF Recombinant Protein PeproTech Cat#250-03-10UG Mouse IL-3 Recombinant Protein PeproTech Cat#213-13-10UG Human IL-6 Recombinant Protein PeproTech Cat#200-06-20UG Human IL-2 Recombinant Protein PeproTech Cat#200-02-50UG Mouse IL-15 Recombinant Protein PeproTech Cat#210-15-10UG Human VEGF-165 Recombinant Protein PeproTech Cat#100-20-10UG Human BMP-4 Recombinant Protein PeproTech Cat#120-05-5UG 2-Deoxy-D-glucose MCE Cat#HY-13966 PKH 67 MCE Cat#HY-D1421 DiR MCE Cat#HY-D1048 Matrigel Matrix Corning Cat#356234 Donkey serum Sigma-Aldrich Cat#D9663 Imidazole Sigma-Aldrich Cat#I202 Ni-NTA Agarose Qiagen Cat#30210 Imidazole Wash Buffer Bioworld Cat#42320000 RIPA buffer Beyotime Cat#P0013E Lenti-X concentrator Takara Cat#631231 methylcellulose Methocult Cat#M3434 Purified CD133 protein This paper N/A 89Zr Qilu hospital N/A Critical commercial assays Annexin V-FITC/PI Apoptosis Kit Biolegend Cat#640914 PKH67GL-1KT Sigma-Aldrich Cat#PKH67GL TUNEL Staining Kit Sigma-Aldrich Cat#APT110 L-Lactate Assay Kit Sigma-Aldrich Cat#MAK329 Glucose Uptake Assay Kit Abcam Cat#ab136956 Cell Counting Kit Yeasen Cat#40203ES60 BCA assay kit Beyotime Cat#P0009 Mouse IFN-γ ELISA Kit Beyotime Cat#PI508 Mycoplasma PCR Detection Kit Beyotime Cat#C0301S 10X Genomics Chromium NextGEM Single Cell 3′ Kit 10× Genomics Cat#PN-1000121 Luciferase Assay System Promega Cat#E1500 Mouse Ccl3 ELISA Kit JONLNBIO Cat#JL20414 (Continued on next page) e2 Cell Reports Medicine 7, 102545, February 17, 2026 Article ll OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Mouse Ccl5 ELISA Kit JONLNBIO Cat#JL17335 Mouse Cxcl1 ELISA Kit JONLNBIO Cat#JL20150 Mouse Cxcl5 ELISA Kit JONLNBIO Cat#JL28925 Mouse IL-1α ELISA Kit JONLNBIO Cat#JL13044 Mouse IL-1β ELISA Kit JONLNBIO Cat#JL18442 Mouse IL-6 ELISA Kit JONLNBIO Cat#JL20268 Mouse Tnf-α ELISA Kit JONLNBIO Cat#JL10484 Mouse Csf3 ELISA Kit JONLNBIO Cat#JL10697 Mouse Cxcl2 ELISA Kit Abnova Cat#KA1505 Deposited data Single-cell sequencing data This paper GEO: GSE293929 Experimental models: Cell lines HEK 293T/17 ATCC CatCRL-11268 K562 ATCC Cat#CCL-243 Panc02 ATCC Cat#CRL-2553 Mouse iPSC Alstem Cat#iPS02M Human iPSC ATCC Cat#ACS-1027 H22 Ubigene Biosciences Cat#YC-C053 Colon26 Creative biogene Cat#CSC-RR0461 Hep-53.4 CLS Cell Lines Service Cat#400200 Panc02-Luc This paper N/A Colon26-Luc This paper N/A Hep-53.4-Luc This paper N/A Experimental models: Organisms/strains F4/80-Cre mice The Jackson laboratory N/A huHSC-NCG-M Gempharmatech Cat#T057533 BALB/c Mice Charles Cat#028 C57BL/6 Mice Charles Cat#027 C57BL/6Smoc-Nos2em1(flox)Smoc Shanghai Model Organisms Cat#NM-CKO-226494 Macrophage-specific Nos2-KO mice This paper N/A Recombinant DNA pCDH-CMV-MCS-EF1α-GreenPuro System Biosciences Cat#CD513B-1 psPAX2 Addgene Cat#12260 pMD2.G Addgene Cat#12259 pLVX-mbIL-21-puro Addgene Cat#125839 Luciferase lentivirus Applied Biological Materials Cat#LVP010079 pET-14B-CD133 This paper N/A Software and algorithms ImageJ Schneider et al.45 https://imagej.net/ij/ Graphpad Prism version 10.0 GraphPad software https://www.graphpad.com FlowJo 10.0 FlowJo, LLC https://www.flowjo.com/ Living Image software v4.5 PerkinElmer N/A SPSS 20.0 IBM https://www.ibm.com/it-it/products/ spss-statistics Biacore S200 Evaluation software Cytiva https://www.cytivalifesciences.com/en/ us/support/software/biacore-downloads/ Biorender Biorender https://app.biorender.com/ Other Nanobodies targeting CD133 This paper N/A Cell Reports Medicine 7, 102545, February 17, 2026 e3 Article ll OPEN ACCESS .. Immunocompetent male C57BL/6 (Cat. 027) and male BALB/c (Cat. 028) mice, aged 8 weeks, were acquired commercially (Charles River Laboratories, IL, USA).

    Enzyme-linked Immunosorbent Assay:

    Article Title: Programmable iPSC-derived CAR-NK vesicles remodel the immune microenvironment and eradicate tumors.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Ultra-LEAFTM Purified anti-mouse IFN-γ Antibody Biolegend Cat#631054; RRID: AB_3097543 Brilliant Violet 421TM anti-mouse CD62L Antibody Biolegend Cat#104435; RRID: AB_10900082 Alexa Fluor® 700 anti-mouse CD44 Antibody Biolegend Cat#156009; RRID: AB_3068217 Anti-mouse CD8β (Lyt 3.2) Selleckchem Cat#A2137; RRID: AB_2687706 InVivoMAb anti-mouse PD-L1 BioXcell Cat#BE0101; RRID: AB_10949073 InVivoMAb anti-mouse CD47 BioXcell Cat#BE0270; RRID: AB_2687793 InVivoMAb anti-mouse CTLA-4 BioXcell Cat#BE0131; RRID: AB_10950184 Bacterial and virus strains BL21(DE3) Sigma-Aldrich 69450 T7 phage Novagen (EMD MIllipore) 70010–3 Chemicals, peptides, and recombinant proteins Puromycin Dihydrochloride Gibco Cat#A1113803 Mouse LIF Recombinant Protein Gibco Cat#PMC9484 Human SCF Recombinant Protein Gibco Cat#PHC2111 MethoCultTM GF M3434 Fisher scientific Cat#NC9099909 Mouse SCF Recombinant Protein PeproTech Cat#250-03-10UG Mouse IL-3 Recombinant Protein PeproTech Cat#213-13-10UG Human IL-6 Recombinant Protein PeproTech Cat#200-06-20UG Human IL-2 Recombinant Protein PeproTech Cat#200-02-50UG Mouse IL-15 Recombinant Protein PeproTech Cat#210-15-10UG Human VEGF-165 Recombinant Protein PeproTech Cat#100-20-10UG Human BMP-4 Recombinant Protein PeproTech Cat#120-05-5UG 2-Deoxy-D-glucose MCE Cat#HY-13966 PKH 67 MCE Cat#HY-D1421 DiR MCE Cat#HY-D1048 Matrigel Matrix Corning Cat#356234 Donkey serum Sigma-Aldrich Cat#D9663 Imidazole Sigma-Aldrich Cat#I202 Ni-NTA Agarose Qiagen Cat#30210 Imidazole Wash Buffer Bioworld Cat#42320000 RIPA buffer Beyotime Cat#P0013E Lenti-X concentrator Takara Cat#631231 methylcellulose Methocult Cat#M3434 Purified CD133 protein This paper N/A 89Zr Qilu hospital N/A Critical commercial assays Annexin V-FITC/PI Apoptosis Kit Biolegend Cat#640914 PKH67GL-1KT Sigma-Aldrich Cat#PKH67GL TUNEL Staining Kit Sigma-Aldrich Cat#APT110 L-Lactate Assay Kit Sigma-Aldrich Cat#MAK329 Glucose Uptake Assay Kit Abcam Cat#ab136956 Cell Counting Kit Yeasen Cat#40203ES60 BCA assay kit Beyotime Cat#P0009 Mouse IFN-γ ELISA Kit Beyotime Cat#PI508 Mycoplasma PCR Detection Kit Beyotime Cat#C0301S 10X Genomics Chromium NextGEM Single Cell 3′ Kit 10× Genomics Cat#PN-1000121 Luciferase Assay System Promega Cat#E1500 Mouse Ccl3 ELISA Kit JONLNBIO Cat#JL20414 (Continued on next page) e2 Cell Reports Medicine 7, 102545, February 17, 2026 Article ll OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Mouse Ccl5 ELISA Kit JONLNBIO Cat#JL17335 Mouse Cxcl1 ELISA Kit JONLNBIO Cat#JL20150 Mouse Cxcl5 ELISA Kit JONLNBIO Cat#JL28925 Mouse IL-1α ELISA Kit JONLNBIO Cat#JL13044 Mouse IL-1β ELISA Kit JONLNBIO Cat#JL18442 Mouse IL-6 ELISA Kit JONLNBIO Cat#JL20268 Mouse Tnf-α ELISA Kit JONLNBIO Cat#JL10484 Mouse Csf3 ELISA Kit JONLNBIO Cat#JL10697 Mouse Cxcl2 ELISA Kit Abnova Cat#KA1505 Deposited data Single-cell sequencing data This paper GEO: GSE293929 Experimental models: Cell lines HEK 293T/17 ATCC CatCRL-11268 K562 ATCC Cat#CCL-243 Panc02 ATCC Cat#CRL-2553 Mouse iPSC Alstem Cat#iPS02M Human iPSC ATCC Cat#ACS-1027 H22 Ubigene Biosciences Cat#YC-C053 Colon26 Creative biogene Cat#CSC-RR0461 Hep-53.4 CLS Cell Lines Service Cat#400200 Panc02-Luc This paper N/A Colon26-Luc This paper N/A Hep-53.4-Luc This paper N/A Experimental models: Organisms/strains F4/80-Cre mice The Jackson laboratory N/A huHSC-NCG-M Gempharmatech Cat#T057533 BALB/c Mice Charles Cat#028 C57BL/6 Mice Charles Cat#027 C57BL/6Smoc-Nos2em1(flox)Smoc Shanghai Model Organisms Cat#NM-CKO-226494 Macrophage-specific Nos2-KO mice This paper N/A Recombinant DNA pCDH-CMV-MCS-EF1α-GreenPuro System Biosciences Cat#CD513B-1 psPAX2 Addgene Cat#12260 pMD2.G Addgene Cat#12259 pLVX-mbIL-21-puro Addgene Cat#125839 Luciferase lentivirus Applied Biological Materials Cat#LVP010079 pET-14B-CD133 This paper N/A Software and algorithms ImageJ Schneider et al.45 https://imagej.net/ij/ Graphpad Prism version 10.0 GraphPad software https://www.graphpad.com FlowJo 10.0 FlowJo, LLC https://www.flowjo.com/ Living Image software v4.5 PerkinElmer N/A SPSS 20.0 IBM https://www.ibm.com/it-it/products/ spss-statistics Biacore S200 Evaluation software Cytiva https://www.cytivalifesciences.com/en/ us/support/software/biacore-downloads/ Biorender Biorender https://app.biorender.com/ Other Nanobodies targeting CD133 This paper N/A Cell Reports Medicine 7, 102545, February 17, 2026 e3 Article ll OPEN ACCESS .. Immunocompetent male C57BL/6 (Cat. 027) and male BALB/c (Cat. 028) mice, aged 8 weeks, were acquired commercially (Charles River Laboratories, IL, USA).

    Single Cell:

    Article Title: Programmable iPSC-derived CAR-NK vesicles remodel the immune microenvironment and eradicate tumors.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Ultra-LEAFTM Purified anti-mouse IFN-γ Antibody Biolegend Cat#631054; RRID: AB_3097543 Brilliant Violet 421TM anti-mouse CD62L Antibody Biolegend Cat#104435; RRID: AB_10900082 Alexa Fluor® 700 anti-mouse CD44 Antibody Biolegend Cat#156009; RRID: AB_3068217 Anti-mouse CD8β (Lyt 3.2) Selleckchem Cat#A2137; RRID: AB_2687706 InVivoMAb anti-mouse PD-L1 BioXcell Cat#BE0101; RRID: AB_10949073 InVivoMAb anti-mouse CD47 BioXcell Cat#BE0270; RRID: AB_2687793 InVivoMAb anti-mouse CTLA-4 BioXcell Cat#BE0131; RRID: AB_10950184 Bacterial and virus strains BL21(DE3) Sigma-Aldrich 69450 T7 phage Novagen (EMD MIllipore) 70010–3 Chemicals, peptides, and recombinant proteins Puromycin Dihydrochloride Gibco Cat#A1113803 Mouse LIF Recombinant Protein Gibco Cat#PMC9484 Human SCF Recombinant Protein Gibco Cat#PHC2111 MethoCultTM GF M3434 Fisher scientific Cat#NC9099909 Mouse SCF Recombinant Protein PeproTech Cat#250-03-10UG Mouse IL-3 Recombinant Protein PeproTech Cat#213-13-10UG Human IL-6 Recombinant Protein PeproTech Cat#200-06-20UG Human IL-2 Recombinant Protein PeproTech Cat#200-02-50UG Mouse IL-15 Recombinant Protein PeproTech Cat#210-15-10UG Human VEGF-165 Recombinant Protein PeproTech Cat#100-20-10UG Human BMP-4 Recombinant Protein PeproTech Cat#120-05-5UG 2-Deoxy-D-glucose MCE Cat#HY-13966 PKH 67 MCE Cat#HY-D1421 DiR MCE Cat#HY-D1048 Matrigel Matrix Corning Cat#356234 Donkey serum Sigma-Aldrich Cat#D9663 Imidazole Sigma-Aldrich Cat#I202 Ni-NTA Agarose Qiagen Cat#30210 Imidazole Wash Buffer Bioworld Cat#42320000 RIPA buffer Beyotime Cat#P0013E Lenti-X concentrator Takara Cat#631231 methylcellulose Methocult Cat#M3434 Purified CD133 protein This paper N/A 89Zr Qilu hospital N/A Critical commercial assays Annexin V-FITC/PI Apoptosis Kit Biolegend Cat#640914 PKH67GL-1KT Sigma-Aldrich Cat#PKH67GL TUNEL Staining Kit Sigma-Aldrich Cat#APT110 L-Lactate Assay Kit Sigma-Aldrich Cat#MAK329 Glucose Uptake Assay Kit Abcam Cat#ab136956 Cell Counting Kit Yeasen Cat#40203ES60 BCA assay kit Beyotime Cat#P0009 Mouse IFN-γ ELISA Kit Beyotime Cat#PI508 Mycoplasma PCR Detection Kit Beyotime Cat#C0301S 10X Genomics Chromium NextGEM Single Cell 3′ Kit 10× Genomics Cat#PN-1000121 Luciferase Assay System Promega Cat#E1500 Mouse Ccl3 ELISA Kit JONLNBIO Cat#JL20414 (Continued on next page) e2 Cell Reports Medicine 7, 102545, February 17, 2026 Article ll OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Mouse Ccl5 ELISA Kit JONLNBIO Cat#JL17335 Mouse Cxcl1 ELISA Kit JONLNBIO Cat#JL20150 Mouse Cxcl5 ELISA Kit JONLNBIO Cat#JL28925 Mouse IL-1α ELISA Kit JONLNBIO Cat#JL13044 Mouse IL-1β ELISA Kit JONLNBIO Cat#JL18442 Mouse IL-6 ELISA Kit JONLNBIO Cat#JL20268 Mouse Tnf-α ELISA Kit JONLNBIO Cat#JL10484 Mouse Csf3 ELISA Kit JONLNBIO Cat#JL10697 Mouse Cxcl2 ELISA Kit Abnova Cat#KA1505 Deposited data Single-cell sequencing data This paper GEO: GSE293929 Experimental models: Cell lines HEK 293T/17 ATCC CatCRL-11268 K562 ATCC Cat#CCL-243 Panc02 ATCC Cat#CRL-2553 Mouse iPSC Alstem Cat#iPS02M Human iPSC ATCC Cat#ACS-1027 H22 Ubigene Biosciences Cat#YC-C053 Colon26 Creative biogene Cat#CSC-RR0461 Hep-53.4 CLS Cell Lines Service Cat#400200 Panc02-Luc This paper N/A Colon26-Luc This paper N/A Hep-53.4-Luc This paper N/A Experimental models: Organisms/strains F4/80-Cre mice The Jackson laboratory N/A huHSC-NCG-M Gempharmatech Cat#T057533 BALB/c Mice Charles Cat#028 C57BL/6 Mice Charles Cat#027 C57BL/6Smoc-Nos2em1(flox)Smoc Shanghai Model Organisms Cat#NM-CKO-226494 Macrophage-specific Nos2-KO mice This paper N/A Recombinant DNA pCDH-CMV-MCS-EF1α-GreenPuro System Biosciences Cat#CD513B-1 psPAX2 Addgene Cat#12260 pMD2.G Addgene Cat#12259 pLVX-mbIL-21-puro Addgene Cat#125839 Luciferase lentivirus Applied Biological Materials Cat#LVP010079 pET-14B-CD133 This paper N/A Software and algorithms ImageJ Schneider et al.45 https://imagej.net/ij/ Graphpad Prism version 10.0 GraphPad software https://www.graphpad.com FlowJo 10.0 FlowJo, LLC https://www.flowjo.com/ Living Image software v4.5 PerkinElmer N/A SPSS 20.0 IBM https://www.ibm.com/it-it/products/ spss-statistics Biacore S200 Evaluation software Cytiva https://www.cytivalifesciences.com/en/ us/support/software/biacore-downloads/ Biorender Biorender https://app.biorender.com/ Other Nanobodies targeting CD133 This paper N/A Cell Reports Medicine 7, 102545, February 17, 2026 e3 Article ll OPEN ACCESS .. Immunocompetent male C57BL/6 (Cat. 027) and male BALB/c (Cat. 028) mice, aged 8 weeks, were acquired commercially (Charles River Laboratories, IL, USA).

    Sequencing:

    Article Title: Programmable iPSC-derived CAR-NK vesicles remodel the immune microenvironment and eradicate tumors.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Ultra-LEAFTM Purified anti-mouse IFN-γ Antibody Biolegend Cat#631054; RRID: AB_3097543 Brilliant Violet 421TM anti-mouse CD62L Antibody Biolegend Cat#104435; RRID: AB_10900082 Alexa Fluor® 700 anti-mouse CD44 Antibody Biolegend Cat#156009; RRID: AB_3068217 Anti-mouse CD8β (Lyt 3.2) Selleckchem Cat#A2137; RRID: AB_2687706 InVivoMAb anti-mouse PD-L1 BioXcell Cat#BE0101; RRID: AB_10949073 InVivoMAb anti-mouse CD47 BioXcell Cat#BE0270; RRID: AB_2687793 InVivoMAb anti-mouse CTLA-4 BioXcell Cat#BE0131; RRID: AB_10950184 Bacterial and virus strains BL21(DE3) Sigma-Aldrich 69450 T7 phage Novagen (EMD MIllipore) 70010–3 Chemicals, peptides, and recombinant proteins Puromycin Dihydrochloride Gibco Cat#A1113803 Mouse LIF Recombinant Protein Gibco Cat#PMC9484 Human SCF Recombinant Protein Gibco Cat#PHC2111 MethoCultTM GF M3434 Fisher scientific Cat#NC9099909 Mouse SCF Recombinant Protein PeproTech Cat#250-03-10UG Mouse IL-3 Recombinant Protein PeproTech Cat#213-13-10UG Human IL-6 Recombinant Protein PeproTech Cat#200-06-20UG Human IL-2 Recombinant Protein PeproTech Cat#200-02-50UG Mouse IL-15 Recombinant Protein PeproTech Cat#210-15-10UG Human VEGF-165 Recombinant Protein PeproTech Cat#100-20-10UG Human BMP-4 Recombinant Protein PeproTech Cat#120-05-5UG 2-Deoxy-D-glucose MCE Cat#HY-13966 PKH 67 MCE Cat#HY-D1421 DiR MCE Cat#HY-D1048 Matrigel Matrix Corning Cat#356234 Donkey serum Sigma-Aldrich Cat#D9663 Imidazole Sigma-Aldrich Cat#I202 Ni-NTA Agarose Qiagen Cat#30210 Imidazole Wash Buffer Bioworld Cat#42320000 RIPA buffer Beyotime Cat#P0013E Lenti-X concentrator Takara Cat#631231 methylcellulose Methocult Cat#M3434 Purified CD133 protein This paper N/A 89Zr Qilu hospital N/A Critical commercial assays Annexin V-FITC/PI Apoptosis Kit Biolegend Cat#640914 PKH67GL-1KT Sigma-Aldrich Cat#PKH67GL TUNEL Staining Kit Sigma-Aldrich Cat#APT110 L-Lactate Assay Kit Sigma-Aldrich Cat#MAK329 Glucose Uptake Assay Kit Abcam Cat#ab136956 Cell Counting Kit Yeasen Cat#40203ES60 BCA assay kit Beyotime Cat#P0009 Mouse IFN-γ ELISA Kit Beyotime Cat#PI508 Mycoplasma PCR Detection Kit Beyotime Cat#C0301S 10X Genomics Chromium NextGEM Single Cell 3′ Kit 10× Genomics Cat#PN-1000121 Luciferase Assay System Promega Cat#E1500 Mouse Ccl3 ELISA Kit JONLNBIO Cat#JL20414 (Continued on next page) e2 Cell Reports Medicine 7, 102545, February 17, 2026 Article ll OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Mouse Ccl5 ELISA Kit JONLNBIO Cat#JL17335 Mouse Cxcl1 ELISA Kit JONLNBIO Cat#JL20150 Mouse Cxcl5 ELISA Kit JONLNBIO Cat#JL28925 Mouse IL-1α ELISA Kit JONLNBIO Cat#JL13044 Mouse IL-1β ELISA Kit JONLNBIO Cat#JL18442 Mouse IL-6 ELISA Kit JONLNBIO Cat#JL20268 Mouse Tnf-α ELISA Kit JONLNBIO Cat#JL10484 Mouse Csf3 ELISA Kit JONLNBIO Cat#JL10697 Mouse Cxcl2 ELISA Kit Abnova Cat#KA1505 Deposited data Single-cell sequencing data This paper GEO: GSE293929 Experimental models: Cell lines HEK 293T/17 ATCC CatCRL-11268 K562 ATCC Cat#CCL-243 Panc02 ATCC Cat#CRL-2553 Mouse iPSC Alstem Cat#iPS02M Human iPSC ATCC Cat#ACS-1027 H22 Ubigene Biosciences Cat#YC-C053 Colon26 Creative biogene Cat#CSC-RR0461 Hep-53.4 CLS Cell Lines Service Cat#400200 Panc02-Luc This paper N/A Colon26-Luc This paper N/A Hep-53.4-Luc This paper N/A Experimental models: Organisms/strains F4/80-Cre mice The Jackson laboratory N/A huHSC-NCG-M Gempharmatech Cat#T057533 BALB/c Mice Charles Cat#028 C57BL/6 Mice Charles Cat#027 C57BL/6Smoc-Nos2em1(flox)Smoc Shanghai Model Organisms Cat#NM-CKO-226494 Macrophage-specific Nos2-KO mice This paper N/A Recombinant DNA pCDH-CMV-MCS-EF1α-GreenPuro System Biosciences Cat#CD513B-1 psPAX2 Addgene Cat#12260 pMD2.G Addgene Cat#12259 pLVX-mbIL-21-puro Addgene Cat#125839 Luciferase lentivirus Applied Biological Materials Cat#LVP010079 pET-14B-CD133 This paper N/A Software and algorithms ImageJ Schneider et al.45 https://imagej.net/ij/ Graphpad Prism version 10.0 GraphPad software https://www.graphpad.com FlowJo 10.0 FlowJo, LLC https://www.flowjo.com/ Living Image software v4.5 PerkinElmer N/A SPSS 20.0 IBM https://www.ibm.com/it-it/products/ spss-statistics Biacore S200 Evaluation software Cytiva https://www.cytivalifesciences.com/en/ us/support/software/biacore-downloads/ Biorender Biorender https://app.biorender.com/ Other Nanobodies targeting CD133 This paper N/A Cell Reports Medicine 7, 102545, February 17, 2026 e3 Article ll OPEN ACCESS .. Immunocompetent male C57BL/6 (Cat. 027) and male BALB/c (Cat. 028) mice, aged 8 weeks, were acquired commercially (Charles River Laboratories, IL, USA).

    Mouse Assay:

    Article Title: Programmable iPSC-derived CAR-NK vesicles remodel the immune microenvironment and eradicate tumors.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Ultra-LEAFTM Purified anti-mouse IFN-γ Antibody Biolegend Cat#631054; RRID: AB_3097543 Brilliant Violet 421TM anti-mouse CD62L Antibody Biolegend Cat#104435; RRID: AB_10900082 Alexa Fluor® 700 anti-mouse CD44 Antibody Biolegend Cat#156009; RRID: AB_3068217 Anti-mouse CD8β (Lyt 3.2) Selleckchem Cat#A2137; RRID: AB_2687706 InVivoMAb anti-mouse PD-L1 BioXcell Cat#BE0101; RRID: AB_10949073 InVivoMAb anti-mouse CD47 BioXcell Cat#BE0270; RRID: AB_2687793 InVivoMAb anti-mouse CTLA-4 BioXcell Cat#BE0131; RRID: AB_10950184 Bacterial and virus strains BL21(DE3) Sigma-Aldrich 69450 T7 phage Novagen (EMD MIllipore) 70010–3 Chemicals, peptides, and recombinant proteins Puromycin Dihydrochloride Gibco Cat#A1113803 Mouse LIF Recombinant Protein Gibco Cat#PMC9484 Human SCF Recombinant Protein Gibco Cat#PHC2111 MethoCultTM GF M3434 Fisher scientific Cat#NC9099909 Mouse SCF Recombinant Protein PeproTech Cat#250-03-10UG Mouse IL-3 Recombinant Protein PeproTech Cat#213-13-10UG Human IL-6 Recombinant Protein PeproTech Cat#200-06-20UG Human IL-2 Recombinant Protein PeproTech Cat#200-02-50UG Mouse IL-15 Recombinant Protein PeproTech Cat#210-15-10UG Human VEGF-165 Recombinant Protein PeproTech Cat#100-20-10UG Human BMP-4 Recombinant Protein PeproTech Cat#120-05-5UG 2-Deoxy-D-glucose MCE Cat#HY-13966 PKH 67 MCE Cat#HY-D1421 DiR MCE Cat#HY-D1048 Matrigel Matrix Corning Cat#356234 Donkey serum Sigma-Aldrich Cat#D9663 Imidazole Sigma-Aldrich Cat#I202 Ni-NTA Agarose Qiagen Cat#30210 Imidazole Wash Buffer Bioworld Cat#42320000 RIPA buffer Beyotime Cat#P0013E Lenti-X concentrator Takara Cat#631231 methylcellulose Methocult Cat#M3434 Purified CD133 protein This paper N/A 89Zr Qilu hospital N/A Critical commercial assays Annexin V-FITC/PI Apoptosis Kit Biolegend Cat#640914 PKH67GL-1KT Sigma-Aldrich Cat#PKH67GL TUNEL Staining Kit Sigma-Aldrich Cat#APT110 L-Lactate Assay Kit Sigma-Aldrich Cat#MAK329 Glucose Uptake Assay Kit Abcam Cat#ab136956 Cell Counting Kit Yeasen Cat#40203ES60 BCA assay kit Beyotime Cat#P0009 Mouse IFN-γ ELISA Kit Beyotime Cat#PI508 Mycoplasma PCR Detection Kit Beyotime Cat#C0301S 10X Genomics Chromium NextGEM Single Cell 3′ Kit 10× Genomics Cat#PN-1000121 Luciferase Assay System Promega Cat#E1500 Mouse Ccl3 ELISA Kit JONLNBIO Cat#JL20414 (Continued on next page) e2 Cell Reports Medicine 7, 102545, February 17, 2026 Article ll OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Mouse Ccl5 ELISA Kit JONLNBIO Cat#JL17335 Mouse Cxcl1 ELISA Kit JONLNBIO Cat#JL20150 Mouse Cxcl5 ELISA Kit JONLNBIO Cat#JL28925 Mouse IL-1α ELISA Kit JONLNBIO Cat#JL13044 Mouse IL-1β ELISA Kit JONLNBIO Cat#JL18442 Mouse IL-6 ELISA Kit JONLNBIO Cat#JL20268 Mouse Tnf-α ELISA Kit JONLNBIO Cat#JL10484 Mouse Csf3 ELISA Kit JONLNBIO Cat#JL10697 Mouse Cxcl2 ELISA Kit Abnova Cat#KA1505 Deposited data Single-cell sequencing data This paper GEO: GSE293929 Experimental models: Cell lines HEK 293T/17 ATCC CatCRL-11268 K562 ATCC Cat#CCL-243 Panc02 ATCC Cat#CRL-2553 Mouse iPSC Alstem Cat#iPS02M Human iPSC ATCC Cat#ACS-1027 H22 Ubigene Biosciences Cat#YC-C053 Colon26 Creative biogene Cat#CSC-RR0461 Hep-53.4 CLS Cell Lines Service Cat#400200 Panc02-Luc This paper N/A Colon26-Luc This paper N/A Hep-53.4-Luc This paper N/A Experimental models: Organisms/strains F4/80-Cre mice The Jackson laboratory N/A huHSC-NCG-M Gempharmatech Cat#T057533 BALB/c Mice Charles Cat#028 C57BL/6 Mice Charles Cat#027 C57BL/6Smoc-Nos2em1(flox)Smoc Shanghai Model Organisms Cat#NM-CKO-226494 Macrophage-specific Nos2-KO mice This paper N/A Recombinant DNA pCDH-CMV-MCS-EF1α-GreenPuro System Biosciences Cat#CD513B-1 psPAX2 Addgene Cat#12260 pMD2.G Addgene Cat#12259 pLVX-mbIL-21-puro Addgene Cat#125839 Luciferase lentivirus Applied Biological Materials Cat#LVP010079 pET-14B-CD133 This paper N/A Software and algorithms ImageJ Schneider et al.45 https://imagej.net/ij/ Graphpad Prism version 10.0 GraphPad software https://www.graphpad.com FlowJo 10.0 FlowJo, LLC https://www.flowjo.com/ Living Image software v4.5 PerkinElmer N/A SPSS 20.0 IBM https://www.ibm.com/it-it/products/ spss-statistics Biacore S200 Evaluation software Cytiva https://www.cytivalifesciences.com/en/ us/support/software/biacore-downloads/ Biorender Biorender https://app.biorender.com/ Other Nanobodies targeting CD133 This paper N/A Cell Reports Medicine 7, 102545, February 17, 2026 e3 Article ll OPEN ACCESS .. Immunocompetent male C57BL/6 (Cat. 027) and male BALB/c (Cat. 028) mice, aged 8 weeks, were acquired commercially (Charles River Laboratories, IL, USA).

    Luciferase:

    Article Title: Programmable iPSC-derived CAR-NK vesicles remodel the immune microenvironment and eradicate tumors.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Ultra-LEAFTM Purified anti-mouse IFN-γ Antibody Biolegend Cat#631054; RRID: AB_3097543 Brilliant Violet 421TM anti-mouse CD62L Antibody Biolegend Cat#104435; RRID: AB_10900082 Alexa Fluor® 700 anti-mouse CD44 Antibody Biolegend Cat#156009; RRID: AB_3068217 Anti-mouse CD8β (Lyt 3.2) Selleckchem Cat#A2137; RRID: AB_2687706 InVivoMAb anti-mouse PD-L1 BioXcell Cat#BE0101; RRID: AB_10949073 InVivoMAb anti-mouse CD47 BioXcell Cat#BE0270; RRID: AB_2687793 InVivoMAb anti-mouse CTLA-4 BioXcell Cat#BE0131; RRID: AB_10950184 Bacterial and virus strains BL21(DE3) Sigma-Aldrich 69450 T7 phage Novagen (EMD MIllipore) 70010–3 Chemicals, peptides, and recombinant proteins Puromycin Dihydrochloride Gibco Cat#A1113803 Mouse LIF Recombinant Protein Gibco Cat#PMC9484 Human SCF Recombinant Protein Gibco Cat#PHC2111 MethoCultTM GF M3434 Fisher scientific Cat#NC9099909 Mouse SCF Recombinant Protein PeproTech Cat#250-03-10UG Mouse IL-3 Recombinant Protein PeproTech Cat#213-13-10UG Human IL-6 Recombinant Protein PeproTech Cat#200-06-20UG Human IL-2 Recombinant Protein PeproTech Cat#200-02-50UG Mouse IL-15 Recombinant Protein PeproTech Cat#210-15-10UG Human VEGF-165 Recombinant Protein PeproTech Cat#100-20-10UG Human BMP-4 Recombinant Protein PeproTech Cat#120-05-5UG 2-Deoxy-D-glucose MCE Cat#HY-13966 PKH 67 MCE Cat#HY-D1421 DiR MCE Cat#HY-D1048 Matrigel Matrix Corning Cat#356234 Donkey serum Sigma-Aldrich Cat#D9663 Imidazole Sigma-Aldrich Cat#I202 Ni-NTA Agarose Qiagen Cat#30210 Imidazole Wash Buffer Bioworld Cat#42320000 RIPA buffer Beyotime Cat#P0013E Lenti-X concentrator Takara Cat#631231 methylcellulose Methocult Cat#M3434 Purified CD133 protein This paper N/A 89Zr Qilu hospital N/A Critical commercial assays Annexin V-FITC/PI Apoptosis Kit Biolegend Cat#640914 PKH67GL-1KT Sigma-Aldrich Cat#PKH67GL TUNEL Staining Kit Sigma-Aldrich Cat#APT110 L-Lactate Assay Kit Sigma-Aldrich Cat#MAK329 Glucose Uptake Assay Kit Abcam Cat#ab136956 Cell Counting Kit Yeasen Cat#40203ES60 BCA assay kit Beyotime Cat#P0009 Mouse IFN-γ ELISA Kit Beyotime Cat#PI508 Mycoplasma PCR Detection Kit Beyotime Cat#C0301S 10X Genomics Chromium NextGEM Single Cell 3′ Kit 10× Genomics Cat#PN-1000121 Luciferase Assay System Promega Cat#E1500 Mouse Ccl3 ELISA Kit JONLNBIO Cat#JL20414 (Continued on next page) e2 Cell Reports Medicine 7, 102545, February 17, 2026 Article ll OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Mouse Ccl5 ELISA Kit JONLNBIO Cat#JL17335 Mouse Cxcl1 ELISA Kit JONLNBIO Cat#JL20150 Mouse Cxcl5 ELISA Kit JONLNBIO Cat#JL28925 Mouse IL-1α ELISA Kit JONLNBIO Cat#JL13044 Mouse IL-1β ELISA Kit JONLNBIO Cat#JL18442 Mouse IL-6 ELISA Kit JONLNBIO Cat#JL20268 Mouse Tnf-α ELISA Kit JONLNBIO Cat#JL10484 Mouse Csf3 ELISA Kit JONLNBIO Cat#JL10697 Mouse Cxcl2 ELISA Kit Abnova Cat#KA1505 Deposited data Single-cell sequencing data This paper GEO: GSE293929 Experimental models: Cell lines HEK 293T/17 ATCC CatCRL-11268 K562 ATCC Cat#CCL-243 Panc02 ATCC Cat#CRL-2553 Mouse iPSC Alstem Cat#iPS02M Human iPSC ATCC Cat#ACS-1027 H22 Ubigene Biosciences Cat#YC-C053 Colon26 Creative biogene Cat#CSC-RR0461 Hep-53.4 CLS Cell Lines Service Cat#400200 Panc02-Luc This paper N/A Colon26-Luc This paper N/A Hep-53.4-Luc This paper N/A Experimental models: Organisms/strains F4/80-Cre mice The Jackson laboratory N/A huHSC-NCG-M Gempharmatech Cat#T057533 BALB/c Mice Charles Cat#028 C57BL/6 Mice Charles Cat#027 C57BL/6Smoc-Nos2em1(flox)Smoc Shanghai Model Organisms Cat#NM-CKO-226494 Macrophage-specific Nos2-KO mice This paper N/A Recombinant DNA pCDH-CMV-MCS-EF1α-GreenPuro System Biosciences Cat#CD513B-1 psPAX2 Addgene Cat#12260 pMD2.G Addgene Cat#12259 pLVX-mbIL-21-puro Addgene Cat#125839 Luciferase lentivirus Applied Biological Materials Cat#LVP010079 pET-14B-CD133 This paper N/A Software and algorithms ImageJ Schneider et al.45 https://imagej.net/ij/ Graphpad Prism version 10.0 GraphPad software https://www.graphpad.com FlowJo 10.0 FlowJo, LLC https://www.flowjo.com/ Living Image software v4.5 PerkinElmer N/A SPSS 20.0 IBM https://www.ibm.com/it-it/products/ spss-statistics Biacore S200 Evaluation software Cytiva https://www.cytivalifesciences.com/en/ us/support/software/biacore-downloads/ Biorender Biorender https://app.biorender.com/ Other Nanobodies targeting CD133 This paper N/A Cell Reports Medicine 7, 102545, February 17, 2026 e3 Article ll OPEN ACCESS .. Immunocompetent male C57BL/6 (Cat. 027) and male BALB/c (Cat. 028) mice, aged 8 weeks, were acquired commercially (Charles River Laboratories, IL, USA).

    other:

    Article Title: POT1 recruits and regulates CST-Polα/primase at human telomeres.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit monoclonal Anti-DYKDDDDK Tag (D6W5B) antibody Cell Signaling Technology Cat#14793; RRID:AB_2217020 Mouse monoclonal Anti-c-Myc (9E10) antibody Sigma-Aldrich Cat#M4439; RRID:AB_439694 Mouse monoclonal Anti-Strep-tag antibody IBA Lifesciences Cat#2-1507-001; RRID:AB_513133 Mouse monoclonal Anti-HA.11 antibody (16B12) Covance Cat#MMS-101R; RRID:AB_291262 Bacterial and virus strains E. coli: MAX Efficiency DH10Bac Competent Cells Thermo Fisher Cat#10361012 E. coli: One Shot TOP10 Chemically Competent E. coli Thermo Fisher Cat#C404003 Chemicals, peptides, and recombinant proteins Cellfectin II Reagent Thermo Fisher Cat#10362100 DMEM Corning Cat#10-013-CV HyClone Bovine Calf Serum (BCS) Cytiva Cat#SH30073.03 L-Glutamine (200 mM) Gibco Cat#25030081 Penicillin-Streptomycin (10,000 U/mL) Gibco Cat#15140122 MEM Non-Essential Amino Acids Solution (100X) Gibco Cat#11140050 Phosphate-Buffered Saline, 1X without calcium and magnesium, pH 7.4 ± 0.1 Corning Cat#21-040-CM cOmplete, EDTA-free Protease Inhibitor Cocktail Roche Cat#11873580001 PhosSTOP Roche Cat#4906845001 Pierce Anti-c-Myc Magnetic Beads Thermo Fisher Cat#88842 Benzonase Nuclease Millipore Sigma Cat#E1014 NuPAGE LDS Sample Buffer (4X) Thermo Fisher Cat#NP0007 Biotinylated peptides Kinexus Bioinformatics Co. N/A ATP, [g-32P] Perkin Elmer Cat#BLU502Z Strep-Tactin Superflow high capacity resin IBA Lifesciences Cat#2-1208-025 Lambda protein phosphatase (expressed and purified in-house from E. coli) N/A UNIPROT: P03772 Fos-Choline-8, Fluorinated, Anagrade Anatrace Cat#F300F T4 Polynucleotide Kinase New England Biolabs Cat#M0201 Hybond-XL hybridization membranes GE Healthcare Cat#GERPN2020S Deposited data Cryo-EM data of CST–Pola/primase recruitment complex Cai et al.15 EMPIAR: 11131 Cryo-EM map of apo CST–POT1/TPP1 complex This paper EMD: 40659 Cryo-EM map of ssDNA–CST–POT1/TPP1 complex This paper EMD: 40660 Coordinates for apo CST–POT1/TPP1 complex This paper PDB: 8SOJ Coordinates for ssDNA–CST–POT1/TPP1 complex This paper PDB: 8SOK Motion-corrected micrographs for apo CST–POT1/TPP1 complex dataset This paper EMPIAR: 12046 Motion-corrected micrographs for ssDNA–CST–POT1/TPP1 complex dataset This paper EMPIAR: 12047 Experimental models: Cell lines HEK 293T/17 ATCC Cat#CRL-11268; RRID:CVCL_1926 Sf9 Thermo Fisher Cat#11496015; RRID:CVCL_0549 Tni Expression Systems Cat#94-002F; RRID:CVCL_RY32 (Continued on next page) ll OPEN ACCESS Cell 187, 1–14.e1–e8, July 11, 2024 e1 Please cite this article in press as: Cai et al., POT1 recruits and regulates CST-Pola/primase at human telomeres, Cell (2024), https://doi.org/ 10.1016/j.cell.2024.05.002 Article



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